Abstract
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I-RPA was developed by combining sample treatment and RPA detection in a single sealed cartridge. •
No cross reaction was found in respiratory tract-associated viruses. •
The low limit of detection was 35 copies/reaction. •
This I-RPA assay is suitable for detection and monitoring of SARS-CoV-2 in the field.
All Keywords
【초록키워드】 Treatment, viruses, SARS-CoV-2, cross reaction, limit of detection, respiratory tract, RPA, cartridge, sealed, 【제목키워드】 SARS-CoV-2, amplification, Nucleic acid detection, polymerase,
【초록키워드】 Treatment, viruses, SARS-CoV-2, cross reaction, limit of detection, respiratory tract, RPA, cartridge, sealed, 【제목키워드】 SARS-CoV-2, amplification, Nucleic acid detection, polymerase,
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I-RPA는 단일 밀봉 카트리지에 시료 처리와 RPA 검출을 결합하여 개발되었습니다. •
호흡기 관련 바이러스에서는 교차 반응이 발견되지 않았습니다. •
검출의 하한은 35카피/반응이었다. •
이 I-RPA 분석은 현장에서 SARS-CoV-2의 탐지 및 모니터링에 적합합니다.