Abstract
The latent dangers of waterborne viral transmission have become a major public health concern. In this study, reduced graphene oxide (rGO)-Fe 3 O 4 nanoparticles were decorated with cetyltrimethylammonium bromide (CTAB) to adsorb severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike pseudovirus and three human enteric viruses (HuNoV, HRV, and HAdV). The successful combination of CTAB with rGO-Fe 3 O 4 was confirmed by transmission electron microscopy, X-ray diffraction, Fourier-transform infrared spectroscopy, zeta potential, Brunner-Emmet-Teller, and vibrating sample magnetometer measurements. The adsorption of HuNoV and HAdV followed pseudo-first-order kinetics, while that of HRV conformed to the pseudo-second-order model. CTAB-functionalized rGO-Fe 3 O 4 exhibited exceptionally high adsorption of HuNoV, HRV, HAdV and SARS-CoV-2 spike pseudovirus, with maximum adsorption capacities of 3.55 × 10 7 , 7.01 × 10 7 , 2.21 × 10 7 and 6.92 × 10 6 genome copies mg -1 , respectively. Moreover, the composite could effectively adsorb the four types of virus particles from coastal, tap, and river water. In addition, concentrating the virions using CTAB functionalized rGO-Fe 3 O 4 composites before qPCR analysis significantly improved the detection limit. The results indicate that viruses are captured on the surface of CTAB functionalized rGO-Fe 3 O 4 composites through electrostatic interactions and the intrinsic adsorption ability of rGO. Overall, CTAB-functionalized rGO-Fe 3 O 4 composites are promising materials for the adsorption and detection of human enteric viruses as well as SARS-CoV-2 from complex aqueous environments.
Keywords: Human enteric viruses; Reduced graphene oxide; SARS-CoV-2 spike pseudovirus; Virion adsorption.
【저자키워드】 Human enteric viruses, Reduced graphene oxide, SARS-CoV-2 spike pseudovirus, Virion adsorption., 【초록키워드】 public health, SARS-CoV-2, coronavirus, Human, Genome, severe acute respiratory syndrome Coronavirus, pseudovirus, Viral transmission, transmission electron microscopy, SARS-CoV-2 spike, electrostatic interactions, Combination, Detection limit, Adsorption, Composite, water, zeta potential, cetyltrimethylammonium bromide, acute respiratory syndrome, X-ray diffraction, acute respiratory syndrome coronavirus, acute respiratory syndrome coronavirus 2, complex, virus particles, infrared spectroscopy, virion, virions, qPCR analysis, HRV, electrostatic interaction, CTAB, Fourier-transform infrared spectroscopy, intrinsic, virus, significantly, addition, reduced, exhibited, virus particle, tap, 【제목키워드】 SARS-CoV-2 spike, virus, reduced,