Abstract
Rapid, straightforward, and massive diagnosis of coronavirus disease 2019 (COVID-19) is one of the more important measures to mitigate the current pandemics. This work reports on an immunosensor to rapidly detect the spike protein from the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The immunosensing device entraps the spike protein linked to angiotensin-converting enzyme host receptor (ACE2) protein in a sandwich between carboxylated magnetic beads functionalized with an anti-spike antibody and an anti-ACE2 antibody, further labeled with streptavidin (poly)horseradish peroxidase (HRP) reporter enzyme. The particles were confined at the surface of screen-printed gold electrodes, whose signal resulting from the interaction of the enzyme with a mediator was recorded in a portable potentiostat. The immunosensor showed a sensitivity of 0.83 μA∗mL/μg and a limit of detection of 22.5 ng/mL of spike protein, with high reproducibility. As a proof-of-concept, it detected commercial spike protein-supplemented buffer solutions, pseudovirions, isolated viral particles and ten nasopharyngeal swab samples from infected patients compared to samples from three healthy individuals paving the way to detect the virus closer to the patient.
Keywords: ACE2; Coronavirus; Immunosensor; Magnetic beads; SARS-CoV-2; Spike protein.
【저자키워드】 SARS-CoV-2, ACE2, coronavirus, spike protein., immunosensor, Magnetic beads, 【초록키워드】 COVID-19, coronavirus disease, Coronavirus disease 2019, spike, antibody, Diagnosis, severe acute respiratory syndrome Coronavirus, virus, Spike protein, Particle, Nasopharyngeal swab, Protein, sensitivity, limit of detection, Pandemics, Rapid, gold, reporter, anti-spike antibody, horseradish peroxidase, Angiotensin-converting enzyme, Interaction, angiotensin, Viral particles, Magnetic beads, peroxidase, mediator, acute respiratory syndrome, Particles, acute respiratory syndrome coronavirus, infected patients, acute respiratory syndrome coronavirus 2, enzyme, host receptor, measure, proof, reproducibility, nasopharyngeal swab sample, magnetic bead, viral particle, buffer, mitigate, Sandwich, pseudovirions, resulting, detect, the patient, the spike protein, infected patient, healthy individual, HRP, was recorded, 【제목키워드】 complex,