Abstract
Early and accurate diagnosis of SARS-CoV-2 was crucial for COVID-19 control and urgently required ultra-sensitive and rapid detection methods. CRISPR-based detection systems have great potential for rapid SARS-CoV-2 detection, but detecting ultra-low viral loads remains technically challenging. Here, we report an ultrasensitive CRISPR/Cas12a-based electrochemical detection system with an electrochemical biosensor, dubbed CRISPR-SPCE, in which the CRISPR ssDNA reporter was immobilized onto a screen-printed carbon electrode. Electrochemical signals are detected due to CRISPR cleavage, giving enhanced detection sensitivity. CRISPR-SPCE enables ultrasensitive SARS-CoV-2 detection, reaching as few as 0.27 copies μL -1 . Moreover, CRISPR-SPCE is also highly specific and inexpensive, providing a fast and simple SARS-CoV-2 assay.
Keywords: CRISPR/Cas12a; CeO(2) nanorods; Detection; SARS-CoV-2; Screen-printed carbon electrode.
【저자키워드】 SARS-CoV-2, detection, CRISPR/Cas12a, CeO(2) nanorods, Screen-printed carbon electrode., 【초록키워드】 COVID-19, CRISPR, SARS-CoV-2 detection, Screen, Viral load, cleavage, reporter, Detection sensitivity, carbon, ssDNA, detection system, required, diagnosis of SARS-CoV-2, 【제목키워드】 Diagnosis,