Abstract
Coronavirus disease (COVID-19) is an infectious disease that has posed a global health challenge caused by the SARS-CoV-2 virus. Early management and diagnosis of SARS-CoV-2 are crucial for the timely treatment, traceability, and reduction of viral spread. We have developed a rapid method using a Graphene-based Field-Effect Transistor (Gr-FET) for the ultrasensitive detection of SARS-CoV-2 Spike S1 antigen (S1-Ag). The in-house developed antispike S1 antibody (S1-Ab) was covalently immobilized on the surface of a carboxy functionalized graphene channel using carbodiimide chemistry. Ultraviolet-visible spectroscopy, Fourier-Transform Infrared Spectroscopy, X-ray Photoelectron Spectroscopy (XPS), Atomic Force Microscopy (AFM), Optical Microscopy, Raman Spectroscopy, Scanning Electron Microscopy (SEM), Enzyme-Linked Immunosorbent Assays (ELISA), and device stability studies were conducted to characterize the bioconjugation and fabrication process of Gr-FET. In addition, the electrical response of the device was evaluated by monitoring the change in resistance caused by Ag-Ab interaction in real time. For S1-Ag, our Gr-FET devices were tested in the range of 1 fM to 1 μM with a limit of detection of 10 fM in the standard buffer. The fabricated devices are highly sensitive, specific, and capable of detecting low levels of S1-Ag.
Keywords: MERS-CoV antigen; SARS-CoV-2; SARS-CoV-2 Spike S1 antigen; field-effect transistor; graphene.
【저자키워드】 SARS-CoV-2, field-effect transistor, Graphene., MERS-CoV antigen, SARS-CoV-2 Spike S1 antigen, 【초록키워드】 COVID-19, Treatment, coronavirus disease, spike, antibody, SARS-CoV-2 virus, Infectious disease, MERS, viral spread, MERS-CoV, ELISA, Antigen, X-ray, Health, stability, enzyme-linked immunosorbent assays, ultraviolet, management, field-effect transistor, limit of detection, Raman spectroscopy, disease, Graphene, Device, Interaction, SEM, real time, AFM, scanning electron microscopy, reduction, Spectroscopy, house, enzyme, infrared spectroscopy, scanning, force, electron, buffer, traceability, transistor, infrared, carbodiimide, Fourier-transform infrared spectroscopy, Optical Microscopy, tested, caused, addition, evaluated, conducted, electrical, diagnosis of SARS-CoV-2, Photoelectron, the SARS-CoV-2 virus, 【제목키워드】 spike, transistor,