Abstract
The coronavirus disease 2019 (COVID-19) pandemic has become an immense global health crisis. However, the lack of efficient and sensitive on-site testing methods limits early detection for timely isolation and intervention. Here, we present a quantitative and ultrasensitive in situ immunoassay technology for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) detection in saliva (QUIT SARS-CoV-2). Our nanoporous membrane resonator generates a rapid oscillating flow to purify and concentrate fully intact SARS-CoV-2 virus in saliva by 40-fold for in situ detection of viral antigens based on chemiluminescent immunoassay within 20 min. This method can not only achieve a detection sensitivity below 100 copies/ml of virus, comparable to the bench-top PCR equipment; it can also improve detection specificity via direct monitoring of viral loads. The integrated portable QUIT SARS-CoV-2 system, which enables rapid and accurate on-site viral screening with a high-throughput sample pooling strategy, can be performed in primary care settings and substantially improve the detection and prevention of COVID-19.
【초록키워드】 COVID-19, coronavirus disease, SARS-CoV-2, Saliva, Coronavirus disease 2019, coronavirus, primary care, pandemic, SARS-CoV-2 virus, Intervention, severe acute respiratory syndrome Coronavirus, virus, Health crisis, immunoassay, specificity, Health, PCR, Early detection, Isolation, membrane, chemiluminescent immunoassay, Quantitative, Detection sensitivity, can not, Viral antigen, acute respiratory syndrome, viral antigens, acute respiratory syndrome coronavirus, acute respiratory syndrome coronavirus 2, viral loads, limit, prevention of COVID-19, IMPROVE, performed, lack, generate, comparable, 【제목키워드】 immunoassay, SARS-CoV-2 detection,