Abstract
A dual recognition biosensor was developed via introducing aptamer strings and molecular imprinting polymer (MIP) for the selective detection of intact SARS-CoV-2 virus based on screen printed carbon electrode (SPCE) modified with nickel-benzene tricarboxylic acid-metal-organic framework (Ni 3 (BTC) 2 MOF) synthesized by in situ growth method, SARS-CoV-2 S protein-specific amino-aptamer and electropolymerization of dopamine (ePDA). The proposed biosensor showed an excellent linear relationship between charge transfer resistance (R ct ) and increase in virus concentration in the range 10 to 10 8 plaque-forming units/mL (PFU/mL) with a low detection limit of 3.3 ± 0.04 PFU/mL and response time of 20 min. Compared with single-element sensors (aptamer or MIP), it showed higher selectivity for the SARS-CoV-2 virus and facilitated detection in real samples.
Keywords: Aptamer; Electrochemical biosensor; In situ Ni3(BTC)2 MOF; Molecularly imprinted polymer; SARS-CoV-2, covid 19.
【저자키워드】 aptamer, electrochemical biosensor, In situ Ni3(BTC)2 MOF, Molecularly imprinted polymer, SARS-CoV-2, covid 19., 【초록키워드】 SARS-CoV-2, S protein, SARS-CoV-2 virus, virus, Polymer, Dopamine, aptamer, Screen, MOF, molecular, SARS-CoV-2 S protein, Detection limit, Concentration, growth, transfer, SARS-CoV-2 S, linear relationship, selective, carbon, MIP, facilitated, increase in, BTC, the SARS-CoV-2 virus, 【제목키워드】 MOF,