Abstract
Coronavirus disease (COVID-19) is a new and highly contagious disease posing a threat to global public health and wreaking havoc around the world. It’s caused by the Coronavirus that causes severe acute respiratory syndrome (SARS-CoV-2). In the current pandemic situation, rapid and accurate SARS-CoV-2 diagnosis on a large scale is critical for early-stage diagnosis. Early detection and monitoring of viral infections can aid in controlling and preventing infection in large groups of people. Accordingly, we developed a sensitive and high-throughput sandwich electrochemiluminescence immunosensor based on antigen detection for COVID-19 diagnosis (the spike protein of SARS-CoV-2). For the spike protein of SARS-CoV-2, the ECL biosensor had a linear range of 10 ng mL -1 to 10 µg mL -1 with a limit of detection of 1.93 ng mL -1 . The sandwich ECL immunosensor could be used in early clinical diagnosis due to its excellent recovery in detecting SARS-CoV-2, rapid analysis (90 min), and ease of use.
Keywords: Au@BSA-luminol; COVID-19; Electrochemiluminescence; SARS-CoV-2; Sandwich immunosensor.
【저자키워드】 COVID-19, SARS-CoV-2, Electrochemiluminescence, Au@BSA-luminol, Sandwich immunosensor., 【초록키워드】 coronavirus disease, viral infection, pandemic, Diagnosis, viral infections, Spike protein, Severe acute respiratory syndrome, Early detection, COVID-19 diagnosis, limit of detection, contagious disease, group, antigen detection, disease, Clinical diagnosis, Critical, ECL, Analysis, Electrochemiluminescence, acute respiratory syndrome, global public health, contagious, Sandwich, luminol, caused, linear, the spike protein, cause, preventing infection, 【제목키워드】 SARS-CoV-2 spike protein, development,