Abstract
Background: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pneumonia is a newly recognized disease, and its diagnosis is primarily confirmed by routine reverse transcriptase -polymerase chain reaction (RT-PCR) detection of SARS-CoV-2.
Methods: However, we report a confirmed case of SARS-CoV-2 pneumonia with a negative routine RT-PCR.
Results: This case was finally diagnosed by nanopore sequencing combined with antibody of SARS-CoV-2. Simultaneously, the ORF and NP gene variations of SARS-CoV-2 were found.
Conclusions: This case highlighted that false-negative results could be present in routine RT-PCR diagnosis, especially with virus variation. Currently, nanopore pathogen sequencing and antibody detection have been found to be effective in clinical diagnosis.
Keywords: SARS-CoV-2; nanopore sequencing; pneumonia; routine RT-PCR; virus variation.
【저자키워드】 SARS-CoV-2, Pneumonia, nanopore sequencing, routine RT-PCR, virus variation, 【초록키워드】 severe acute respiratory syndrome coronavirus 2, coronavirus, antibody, Variation, Nanopore, Sequencing, Diagnosis, severe acute respiratory syndrome Coronavirus, RT-PCR, virus, Antibody detection, pathogen, nanopore sequencing, SARS-CoV-2 pneumonia, false-negative results, disease, Clinical diagnosis, false-negative, reverse transcriptase, Chain Reaction, acute respiratory syndrome, acute respiratory syndrome coronavirus, transcriptase, RT-PCR diagnosis, effective, diagnosed, ORF, 【제목키워드】 Pneumonia, reaction, chain, routine, negative, Guangzhou, China, Confirmed,