An optimized, amplicon-based approach for sequencing of SARS-CoV-2 from patient samples using COVIDSeq assay on Illumina MiSeq sequencing platforms

Published on
Journal: STAR protocols
[저자] Rahul C Bhoyar, Vigneshwar Senthivel, Bani Jolly, Mohamed Imran, Abhinav Jain, Mohit Kumar Divakar, Vinod Scaria, Sridhar Sivasubbu
[Category] MERS, SARS,
PMC URL    Pubmed URL    [DOI] 10.1016/j.xpro.2021.100755    
[Source] PMC

Abstract
Sequencing of SARS-CoV-2 genomes is crucial for understanding the genetic epidemiology of the COVID-19 pandemic. It is also critical for understanding the evolution of the virus and also for the rapid development of diagnostic tools. The present protocol is a modification of the Illumina COVIDSeq test. We describe an amplicon-based next-generation sequencing approach with short turnaround time, adapted for bench-top sequencers like MiSeq, iSeq, and MiniSeq. For complete details on the use and execution of this protocol, please refer to Bhoyar et al. (2021).
Keywords: Bioinformatics; Clinical Protocol; Genomics; Immunology; Microbiology; Sequence analysis; Sequencing.

All Keywords
【저자키워드】 immunology, Microbiology, Sequencing, bioinformatics, Genomics, Sequence analysis, clinical protocol,