Mass spectrometry and proteome analysis to identify SARS-CoV-2 protein from COVID-19 patient swab samples

Published on
Journal: STAR protocols
[저자] Arghya Banerjee, Medha Gayathri J Pai, Avinash Singh, Mehar Un Nissa, Sanjeeva Srivastava
[Category] COVID19(2023년), MERS, SARS,
PMC URL    Pubmed URL    [DOI] 10.1016/j.xpro.2022.101177    
[Source] PMC

Abstract
With new emerging SARS-CoV-2 strains and their increased pathogenicity, diagnosis has become more challenging. Molecular diagnosis often involves the use of nasopharyngeal swabs and subsequent real-time PCR-based tests. Although this test is the gold standard, it has several limitations; therefore, more complementary assays are required. This protocol describes how to identify SARS-CoV-2 protein from patients’ nasopharyngeal swab samples. We first introduce the approach of label-free quantitative proteomics. We then detail target verification by triple quadrupole mass spectrometry (MS)-based targeted proteomics. For complete details on the use and execution of this profile, please refer to Bankar et al. (2021).
Keywords: Clinical Protocol; Health Sciences; Mass Spectrometry; Protein Biochemistry; Proteomics.

All Keywords
【저자키워드】 proteomics, mass spectrometry, Health Sciences, clinical protocol, Protein Biochemistry,