Transcriptome profiling of PBMCs and formalin-fixed autopsy tissues from COVID-19 patients

Published on
Journal: STAR protocols
[저자] Marta Vuerich, Na Wang, Ahmadreza Kalbasi, Jonathon J Graham, Maria Serena Longhi
[Category] MERS, SARS,
PMC URL    Pubmed URL    [DOI] 10.1016/j.xpro.2022.101156    
[Source] PMC

Abstract
Here we present an optimized protocol for transcriptome profiling of COVID-19 patient samples, including peripheral blood mononuclear cells (PBMCs) and formalin-fixed paraffin-embedded tissue samples obtained from the lung, liver, heart, kidney, and spleen, with the matched controls. We describe RNA extraction and subsequent transcriptome analysis using NanoString technology of the patient samples. The protocol provides information about sample preparation, RNA extraction, and NanoString profiling and analysis. It can be also applied to differentiated Th17 and Treg subsets or formalin-fixed colon tissue samples. For complete details on the use and execution of this protocol, please refer to Wang et al. (2021).
Keywords: Cell Biology; Cell isolation; Cell-based Assays; Clinical Protocol; Gene Expression; Health Sciences; Immunology; Microbiology; Molecular Biology.

All Keywords
【저자키워드】 immunology, Microbiology, Molecular biology, Gene Expression, Cell Biology, Cell isolation, Cell-based Assays, Health Sciences, clinical protocol,