Abstract
A specific aptameric sequence has been immobilized on short polyethyleneglycol (PEG) interface on gold nano-film deposited on a D-shaped plastic optical fiber (POFs) probe, and the protein binding has been monitored exploiting the very sensitive surface plasmon resonance (SPR) phenomenon. The receptor-binding domain (RBD) of the SARS-CoV-2 spike glycoprotein has been specifically used to develop an aptasensor. Surface analysis techniques coupled to fluorescence microscopy and plasmonic analysis have been utilized to characterize the biointerface. Spanning a wide protein range (25 ÷ 1000 nM), the SARS-Cov-2 spike protein was detected with a Limit of Detection (LoD) of about 37 nM. Different interferents (BSA, AH1N1 hemagglutinin protein and MERS spike protein) have been tested confirming the specificity of our aptasensor. Finally, a preliminary test in diluted human serum encouraged its application in a point-of-care device, since POF-based aptasensor represent a potentially low-cost compact biosensor, characterized by a rapid response, a small size and could be an ideal laboratory portable diagnostic tool.
Keywords: Aptamer; Plastic optical fiber (POF); Polyethyleneglycol (PEG); Sars-CoV-2; Self assembled monolayer (SAM); Surface plasmon resonance (SPR).
【저자키워드】 SARS-CoV-2, aptamer, surface plasmon resonance (SPR), Plastic optical fiber (POF), Polyethyleneglycol (PEG), Self assembled monolayer (SAM), 【초록키워드】 spike glycoprotein, diagnostic, detection, MERS, surface plasmon resonance, SPR, Spike protein, Laboratory, aptamer, Protein, point-of-care, specificity, fluorescence microscopy, Receptor-binding domain, RBD, plastic, SARS-CoV-2 spike glycoprotein, SARS-CoV-2 spike protein, gold, BSA, optical, surface, film, human serum, Optical fiber, Protein binding, binding, Analysis, rapid response, surface plasmon, self, domain, sequence, probe, fiber, MERS spike protein, tested, develop, characterized, the SARS-CoV-2, 【제목키워드】 optical, fiber,