Abstract
Fast, efficient, and accurate detection of SARS-CoV-2 spike antigen is pivotal to control the spread and reduce the mortality of COVID-19. Nevertheless, the sensitivity of available nanobiosensors to detect recombinant SARS-CoV-2 spike antigen seems insufficient. As a proof-of-concept, MOF-5/CoNi 2 S 4 is developed as a low-cost, safe, and bioactive hybrid nanostructure via the one-pot high-gravity protocol. Then, the porphyrin, H 2 TMP, was attached to the surface of the synthesized nanomaterial to increase the porosity for efficient detection of recombinant SARS-CoV-2 spike antigen. AFM results approved roughness in different ranges, including 0.54 to 0.74 μm and 0.78 to ≈0.80 μm, showing good physical interactions with the recombinant SARS-CoV-2 spike antigen. MTT assay was performed and compared to the conventional synthesis methods, including hydrothermal, solvothermal, and microwave-assisted methods. The synthesized nanodevices demonstrated above 88% relative cell viability after 24 h and even 48 h of treatment. Besides, the ability of the synthesized nanomaterials to detect the recombinant SARS-CoV-2 spike antigen was investigated, with a detection limit of 5 nM. The in-situ synthesized nanoplatforms exhibited low cytotoxicity, high biocompatibility, and appropriate tunability. The fabricated nanosystems seem promising for future surveys as potential platforms to be integrated into biosensors.
Keywords: Biosensor; COVID-19; High-gravity technique; MOF-5; Recombinant SARS-CoV-2 spike antigen.
【저자키워드】 COVID-19, biosensor, High-gravity technique, MOF-5, Recombinant SARS-CoV-2 spike antigen., 【초록키워드】 Treatment, protocol, Mortality, cytotoxicity, Spread, biosensor, sensitivity, viability, platform, Porphyrin, Interaction, Detection limit, recombinant, AFM, MOF-5, Safe, SARS-CoV-2 spike antigen, proof, physical, Cell, MTT, porosity, detect, investigated, approved, was performed, exhibited, demonstrated, reduce, 【제목키워드】 optical,