Abstract
To prevent the spread of SARS-CoV-2 in cold-chain transportation in China, we developed specific cryogenic disinfectants. Carrier tests were performed against SARS-CoV-2 at – 20 °C for the four cryogenic disinfectants developed and qRT-PCR was used to test the virus RNA. Peracetic acid, chlorine disinfectants (two different concentrations), and quaternary ammonium disinfectant with their antifreeze can all inactivate SARS-CoV-2 in 5 min at – 20 °C. However, after 2-3 h of exposure, only chlorine disinfectant could destroy SARS-CoV-2 RNA. The viruses treated with peracetic acid and quaternary disinfectants showed positive Ct values even after 3 h detected with qRT-PCR. The conclusion was that the cold-chain disinfectants we tested could inactivate SARS-CoV-2 quickly and effectively, but only chlorine disinfectants could destroy nucleic acids in 3 h. Our study also illustrated that using qRT-PCR detection of viral nucleic acids to assess disinfection was inappropriate.
Keywords: Cold chain; Cryogenic disinfectants; Inactivate; SARS-CoV-2; Viral nucleic acids.
【저자키워드】 SARS-CoV-2, cold chain, Cryogenic disinfectants, Inactivate, Viral nucleic acids., 【초록키워드】 qRT-PCR, Spread, China, nucleic acid, nucleic acids, Disinfection, SARS-CoV-2 RNA, cold chain, Ammonium, Cold, Ct values, chlorine, viral nucleic acid, positive, virus RNA, viral nucleic acids, carrier, peracetic acid, Prevent, concentrations, tested, performed, was used, virus, treated, 【제목키워드】 disinfectant,