Abstract
Background: The reprocessing of medical devices has become more complex due to increasing hygiene requirements. Previous studies showed satisfactory bactericidal disinfection effects of UV-C light in rigid and flexible endoscopes. Especially in the context of the current COVID-19 pandemic, virucidal properties are of high importance. In the present study, the virucidal efficacy of UV-C light surface disinfection was analyzed.
Methods: MS-2 bacteriophages were applied to the test samples and irradiated by UV-C light using the UV Smart D25 device; unirradiated test samples were used as controls. A dilution series of the samples was mixed with 1 × 108  Escherichia coli and assayed.
Results: 8.6 × 10 12 pfu could be harvested from the unprocessed test samples. In the control group without UV-C exposure, a remaining contamination of 1.2 × 10 12 pfu was detected, resulting in a procedural baseline reduction rate with a LOG 10 reduction factor of 0.72. The LOG 10 reduction factor was found to be 3.0 after 25 s of UV-C light exposure. After 50 and 75 s of UV-C radiation LOG 10 reduction factors 4.2 and 5.9, respectively, were found, with all reductions being statistically significantly different to baseline.
Conclusions: The tested UV system seems to provide a significant virucidal effect after a relatively short irradiation time.
Keywords: COVID-19; D25; MS-2; UV light; adenovirus; disinfection; endoscopes; otorhinolaryngology; surface disinfection; virus.
【저자키워드】 COVID-19, Adenovirus, Disinfection, Otorhinolaryngology, virus., D25, MS-2, UV light, endoscopes, surface disinfection, 【초록키워드】 Efficacy, COVID-19 pandemic, virus, Adenovirus, Contamination, virucidal, Disinfection, bacteriophage, Otorhinolaryngology, UV light, Escherichia coli, Medical devices, hygiene, reduction, Radiation, Factor, control group, Previous studies, complex, previous study, dilution series, SmaRT, Effect, controls, flexible, resulting, tested, analyzed, significantly, applied, were used, statistically, baseline, dilution sery, pfu, 【제목키워드】 Model, bacteriophage, surface,