Abstract
As an important component of the COVID-19 mRNA vaccines, liposomes play a key role in the efficient protection and delivery of mRNA to cells. Herein, due to the controllable release amplification strategy of liposomes, a reliable and robust single-particle collision electrochemical (SPCE) biosensor was constructed for H9N2 avian influenza virus (H9N2 AIV) detection by combining liposome encapsulation-release strategy with immunomagnetic separation. The liposomes modified with biotin and loaded with platinum nanoparticles (Pt NPs) were used as signal probes for the first time. Biotin facilitated the coupling of biomolecules (DNA or antibodies) through the specific reaction of biotin-streptavidin. Each liposome can encapsulate multiple Pt NPs, which were ruptured under the presence of 1 × PBST (phosphate buffer saline with 0.05% Tween-20) within 2 min, and the encapsulated Pt NPs were released for SPCE experiment. The combination of immunomagnetic separation not only improved the anti-interference capabilities but also avoided the agglomeration of Pt NPs, enabling the SPCE biosensor to realize ultrasensitive detection of 18.1 fg/mL H9N2 AIV. Furthermore, the reliable SPCE biosensor was successfully applied in specific detection of H9N2 AIV in complex samples (chicken serum, chicken liver and chicken lung), which promoted the universality of SPCE biosensor and its application prospect in early diagnosis of diseases.
Keywords: H9N2 AIV; Liposome; Magnetic beads; Single-particle collision electrochemical biosensor; Ultramicroelectrode.
【저자키워드】 Liposome, Magnetic beads, H9N2 AIV, Single-particle collision electrochemical biosensor, Ultramicroelectrode., 【초록키워드】 antibodies, Diseases, Influenza, lung, Influenza virus, Saline, early diagnosis, amplification, DNA, serum, cells, mRNA, experiment, liver, Combination, Biotin, Liposome, Magnetic beads, phosphate, Avian Influenza, Platinum, COVID-19 mRNA vaccines, complex, coupling, probe, streptavidin, robust, phosphate buffer, Tween-20, Ultramicroelectrode, applied, were used, facilitated, released, promoted, encapsulate, PBST, 【제목키워드】 amplification,