Abstract
Here, we reported the synthesis of reduced porous graphene oxide (rPGO) decorated with gold nanoparticles (Au NPs) to modify the ITO electrode. Then we used this highly uniform Au NPs@rPGO modified ITO electrode as a surface-enhanced Raman spectroscopy-active surface and a working electrode. The uses of the Au nanoparticles and porous graphene enhance the Raman signals and the electrochemical conductivity. COVID-19 protein-based biosensor was developed based on immobilization of anti-COVID-19 antibodies onto the modified electrode and its uses as a probe for capturing the COVID-19 protein. The developed biosensor showed the capability of monitoring the COVID-19 protein within a concentration range from 100 nmol/L to 1 pmol/L with a limit of detection (LOD) of 75 fmol/L. Furthermore, COVID-19 protein was detected based on electrochemical techniques within a concentration range from 100 nmol/L to 500 fmol/L that showed a LOD of 39.5 fmol/L. Finally, three concentrations of COVID-19 protein spiked in human serum were investigated. Thus, the present sensor showed high efficiency towards the detection of COVID-19.
Keywords: COVID-19; Gold nanoparticles; Reduced porous graphene oxide modified ITO electrode; Spectroelectrochemical biosensor; Square wave voltammetry; Surface enhanced-Raman spectroscopy.
【저자키워드】 COVID-19, Gold nanoparticles, Reduced porous graphene oxide modified ITO electrode, Spectroelectrochemical biosensor, Square wave voltammetry, Surface enhanced-Raman spectroscopy., 【초록키워드】 antibody, Protein, limit of detection, gold, surface, Raman spectroscopy, immobilization, synthesis, human serum, Graphene, Gold nanoparticle, Concentration, Efficiency, probe, Square wave, ENhance, reported, investigated, reduced, modify, porous, 【제목키워드】 SARS-CoV-2 spike protein, porous,