Abstract
Coronavirus disease 2019 (COVID-19), caused by SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) is a highly contagious disease. To tame the continuously raging outbreak of COVID-19, developing a cheap, rapid and sensitive testing assay is absolutely imperative. Herein, we developed a one-tube colorimetric RT-LAMP assay for the visual detection of SARS-CoV-2 RNA. The assay integrates Si-OH magnetic beads (MBs)-based fast RNA extraction and rapid isothermal amplification in a single tube, thus bypassing the RNA elution step and directly amplifying on-beads RNA molecules with the visualized results. This one-tube assay has a limit of detection (LOD) as low as 200 copies/mL for sample input volumes of up to 600 μL, and can be performed in less than 1 h from sample collection to result readout. This assay demonstrated a 100% concordance with the gold standard test RT-qPCR test by using 29 clinical specimens and showed high specificity. This one-tube colorimetric RT-LAMP assay can serve as an alternative platform for a rapid and sensitive diagnostic test for COVID-19 and is particularly suitable for use at community clinics or township hospitals.
Keywords: LAMP; One-tube; Point-of-care test; SARS-CoV-2; Visual readout.
【저자키워드】 SARS-CoV-2, LAMP, point-of-care test, One-tube, Visual readout., 【초록키워드】 COVID-19, coronavirus disease, Coronavirus disease 2019, coronavirus, diagnostic test, diagnostic, severe acute respiratory syndrome Coronavirus, clinics, hospitals, RNA, RNA extraction, RT-LAMP, specificity, Concordance, outbreak, LAMP, limit of detection, Community, gold, isothermal amplification, SARS-CoV-2 RNA, contagious disease, disease, platform, Visual, Volume, acute respiratory syndrome, acute respiratory syndrome coronavirus, acute respiratory syndrome coronavirus 2, gold standard, specimen, sample collection, contagious, RT-qPCR test, magnetic bead, performed, caused, amplifying, less, demonstrated, imperative, RNA molecule, bypassing, 【제목키워드】 RT-LAMP, Rapid,