Background: Coronavirus (CoV) is an emerging human pathogen causing severe acute respiratory syndrome (SARS) around the world. Earlier identification of biomarkers for SARS can facilitate detection and reduce the mortality rate of the disease. Thus, by integrated network analysis and structural modeling approach, we aimed to explore the potential drug targets and the candidate drugs for coronavirus medicated SARS. Methods: Differentially expression (DE) analysis of CoV infected host genes (HGs) expression profiles was conducted by using the Limma. Highly integrated DE-CoV-HGs were selected to construct the protein-protein interaction (PPI) network. Results: Using the Walktrap algorithm highly interconnected modules include module 1 (202 nodes); module 2 (126 nodes) and module 3 (121 nodes) modules were retrieved from the PPI network. MYC, HDAC9, NCOA3, CEBPB, VEGFA, BCL3, SMAD3, SMURF1, KLHL12, CBL, ERBB4, and CRKL were identified as potential drug targets (PDTs), which are highly expressed in the human respiratory system after CoV infection. Functional terms growth factor receptor binding, c-type lectin receptor signaling, interleukin-1 mediated signaling, TAP dependent antigen processing and presentation of peptide antigen via MHC class I, stimulatory T cell receptor signaling, and innate immune response signaling pathways, signal transduction and cytokine immune signaling pathways were enriched in the modules. Protein-protein docking results demonstrated the strong binding affinity (-314.57 kcal/mol) of the ERBB4-3cLpro complex which was selected as a drug target. In addition, molecular dynamics simulations indicated the structural stability and flexibility of the ERBB4-3cLpro complex. Further, Wortmannin was proposed as a candidate drug to ERBB4 to control SARS-CoV-2 pathogenesis through inhibit receptor tyrosine kinase-dependent macropinocytosis, MAPK signaling, and NF-kb singling pathways that regulate host cell entry, replication, and modulation of the host immune system. Conclusion: We conclude that CoV drug target “ERBB4” and candidate drug “Wortmannin” provide insights on the possible personalized therapeutics for emerging COVID-19.
【저자키워드】 COVID-19, SARS-CoV-2, Biological network analysis, drug targets, ERBB4, growth factor receptor binding, Limma, protein-protein docking, signal transduction pathways, Walktrap algorithm, wortmannin, 【초록키워드】 coronavirus, Biomarker, Biomarkers, innate immune response, interleukin-1, peptide, cytokine, molecular dynamics, molecular dynamics simulations, ERBB4, Limma, wortmannin, binding affinity, Molecular dynamics simulation, Antigen, Replication, Severe acute respiratory syndrome, T cell, interleukin, stability, Algorithm, pathway, Smad3, drug target, network analysis, CoV, MHC class I, Respiratory system, receptor, mortality rate, respiratory, T cell receptor, expression, signaling pathways, SARS-CoV-2 pathogenesis, protein-protein interaction, Signal transduction, flexibility, Signaling, MYC, Analysis, PPI, MAPK, regulate, Pathways, Receptor binding, tyrosine, host cell, acute respiratory syndrome, modulation, complex, CoV infection, module, host immune system, human pathogen, expression profiles, antigen processing, candidate drugs, growth factor, PPI network, BCL3, CEBPB, CRKL, HDAC9, KLHL12, MAPK signaling, NCOA3, SMURF1, VEGFA, approach, expression profile, macropinocytosis, selected, include, indicated, addition, inhibit, conducted, facilitate, expressed, demonstrated, reduce, host gene, stimulatory, candidate drug, CBL, docking result, immune signaling pathway, medicated, receptor signaling, retrieved, was selected, 【제목키워드】 identifying, approach, candidate drug,