Abstract
Wastewater-Based Epidemiology is a tool to face and mitigate COVID-19 outbreaks by evaluating conditions in a specific community. This study aimed to analyze the microbiome profiles using nanopore technology for full-length 16S rRNA sequencing in wastewater samples collected from a penitentiary (P), a residential care home (RCH), and a quarantine or health care facilities (HCF). During the study, the wastewater samples from the RCH and the P were negative for SARS-CoV-2 based on qPCRs, except during the fourth week when was detected. Unexpectedly, the wastewater microbiome from RCH and P prior to week four was correlated with the samples collected from the HCF, suggesting a core bacterial community is expelled from the digest tract of individuals infected with SARS-CoV-2. The microbiota of wastewater sample positives for SARS-CoV-2 was strongly associated with enteric bacteria previously reported in patients with risk factors for COVID-19. We provide novel evidence that the wastewater microbiome associated with gastrointestinal manifestations appears to precede the SARS-CoV-2 detection in sewage. This finding suggests that the wastewaters microbiome can be applied as an indicator of community-wide SARS-CoV-2 surveillance.
Keywords: Microbiome; Nanopore sequencing; SARS-CoV-2; Wastewater.
【저자키워드】 SARS-CoV-2, wastewater, Microbiome, nanopore sequencing, 【초록키워드】 COVID-19, Risk factors, Health care, quarantine, Sequencing, risk factor, 16S rRNA, SARS-CoV-2 detection, wastewater, Health, microbiota, COVID-19 outbreak, Patient, Microbiome, Community, nanopore sequencing, Bacteria, sewage, Care, rRNA, Bacterial, Evidence, SARS-CoV-2 surveillance, manifestation, residential care, Indicator, profile, individual, positive, full-length, nanopore technology, mitigate, collected, reported, appear, condition, correlated, expelled, infected with SARS-CoV-2, the SARS-CoV-2, 【제목키워드】 COVID-19, Surveillance,