Abstract SARS-CoV-2 has spread rapidly worldwide, causing the COVID-19 pandemic. Platelet activation and platelet-leukocyte complex formation are proposed to contribute to disease progression. Here, we report platelet and leukocyte activation during acute and convalescent COVID-19 in patients recruited between May-July 2020. Blood samples were analyzed by flow cytometry and ELISA using paired comparison between inclusion (day 0) and 28 days later. The majority of patients were mildly or moderately ill with significantly higher cytokine levels (IL-6 and IL-10) on day 0 as compared with day 28. Platelet activation and granule release were significantly higher on day 0 compared with day 28, as determined by ADP- or thrombin-induced surface CD62P expression, baseline released CD62P, and thrombin-induced platelet-monocyte complex formation. Monocyte activation and procoagulant status at baseline and post activation were heterogeneous but generally lower on day 0 compared with day 28. Baseline and thrombin- or fMLF-induced neutrophil activation and procoagulant status were significantly lower on day 0 compared with day 28. We demonstrate that during the acute phase of COVID-19 compared with the convalescent phase, platelets are more responsive while neutrophils are less responsive. COVID-19 is associated with thromboembolic events where platelet activation and interaction with leukocytes may play an important role.
【저자키워드】 Blood platelets, platelet activation, 【초록키워드】 COVID-19, Neutrophils, SARS-CoV-2, platelet activation, IL-6, COVID-19 pandemic, neutrophil, Platelets, cytokine, flow cytometry, ELISA, monocyte, Spread, Disease progression, Patient, Platelet, IL-10, convalescent, expression, leukocytes, CD62P, Interaction, neutrophil activation, thrombin, determined by, leukocyte, thromboembolic, Activation, Thromboembolic events, thrombin-, Blood samples, acute phase, convalescent phase, leukocyte activation, Monocyte activation, platelet-leukocyte complex formation, platelet-monocyte complex formation, procoagulant status, blood sample, heterogeneous, significantly lower, Inclusion, Thromboembolic event, analyzed, recruited, majority, less, contribute, significantly higher, released, ADP, baseline, COVID-19 in patient, cytokine level, 【제목키워드】 COVID-19, phenotype, occur,