Abstract
Conventional methods for quantifying and phenotyping antigen-specific lymphocytes can rapidly deplete irreplaceable specimens. This is due to the fact that antigen-specific T and B cells have historically been analyzed in independent assays each requiring millions of cells. A technique that facilitates the simultaneous detection of antigen-specific T and B cells would allow for more thorough immune profiling with significantly reduced sample requirements. To this end, we developed the B and T cell tandem lymphocyte evaluation (BATTLE) assay, which allows for the simultaneous identification of SARS-CoV-2 Spike reactive T and B cells using an activation induced marker (AIM) T cell assay and dual-color B cell antigen probes. Using this assay, we demonstrate that antigen-specific B and T cell subsets can be identified simultaneously using conventional flow cytometry platforms and provide insight into the differential effects of mRNA vaccination on B and T cell populations following natural SARS-CoV-2 infection.
Keywords: B cells; SARS-CoV-2; T cells; antigen-specific; natural infection; spike protein; vaccination.
【저자키워드】 SARS-CoV-2, vaccination, T cells, B cells, Spike protein, natural infection, antigen-specific, 【초록키워드】 mRNA vaccination, vaccination, Lymphocytes, spike, T cells, SARS-COV-2 infection, B cells, flow cytometry, Population, Spike protein, Antigen, B cell, lymphocyte, T cell, cells, immune profiling, natural infection, specimens, platform, marker, T and B cells, Activation, technique, independent assays, probes, Effect, reactive, analyzed, significantly, reduced, facilitate, deplete, T cell subset, Conventional, independent assay, 【제목키워드】 vaccination, T cells, Analysis, Simultaneous,