Anopheles freeborni mosquitoes were infected by feeding on a New World monkey, Aotus trivirgatus, infected with the Malayan IV strain of Plasmodium falciparum. After a normal incubation period, the infection was passed to a human volunteer through the bites of these mosquitoes, demonstrating for the first time the practicability of using a simian host as a donor for the infection of mosquitoes with this species of human malarial parasites.
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