Abstract
In this work, a brief electrochemical aptasensor was developed for highly sensitive detection of SARS-CoV-2 antigen utilizing an aptamer-binding induced multiple hairpin assembly strategy for signal amplification. In the presence of SARS-CoV-2, a pair of aptamers was brought in a close proximity according to the aptamer-protein antigen binding, which initiated strand displacement reaction thereby triggering a multiple hairpin assembly to obtain long linear DNA concatemers on the electrode surface. As the fabricated hairpin probes were labeled with biotin, massive streptavidin-alkaline phosphatases (ST-ALP) could be further introduced on the electrode interface via biotin-streptavidin interaction thus generating strong electrochemical signal in electrolyte solution containing 1-naphthol phosphate. Benefiting from the non-enzymatic multiple hairpin assembly signal amplification strategy, the designed aptasensor for SARS-CoV-2 spike protein detection exhibited the wide linear range from 50 fg·mL -1 to 50 ng·mL -1 and low detection limit of 9.79 fg·mL -1 . Meaningfully, this proposed electrochemical assay provided a potential application for the point of care analysis of viral diseases under ambient temperature.
Keywords: Aptamer/protein proximity binding; Electrochemical aptasensor; Multiple hairpin assembly; SARS-CoV-2; Spike protein.
【저자키워드】 SARS-CoV-2, spike protein., Electrochemical aptasensor, Aptamer/protein proximity binding, Multiple hairpin assembly, 【초록키워드】 spike, point of care, Spike protein, Antigen, Protein, amplification, DNA, SARS-CoV-2 spike protein, temperature, viral disease, SARS-CoV-2 antigen, Viral diseases, binding, Interaction, Detection limit, Biotin, Analysis, phosphate, phosphatase, ambient temperature, antigen binding, pair, Multiple, triggering, probe, ambient, 1-NAPHTHOL, provided, linear, exhibited, introduced, initiated, 【제목키워드】 amplification,