Abstract
We present a course-embedded undergraduate research module that involves real-time polymerase chain reaction testing for the presence of SARS-CoV-2 in environmental samples. A positive control RNA was constructed and two RNA extraction methods were compared and a range of primers were available to compare. Using a combination of published protocols, we assembled a successful project that illustrated a topical exercise similar to real-world assay development. The exercise is aimed at upper-level undergraduates and requires 3 weeks of laboratory periods. The students were able to design and test experimental protocols, while learning about RNA detection. This project could be utilized in upper-level classes including molecular biology, biochemistry, biotechnology, or for independent research projects.
Keywords: RT-PCR; SARS-CoV2; laboratory exercise.
【저자키워드】 SARS-CoV2, RT-PCR, laboratory exercise., 【초록키워드】 SARS-CoV-2, exercise, Biochemistry, Molecular biology, Biotechnology, RT-PCR, Laboratory, RNA, RNA extraction, polymerase chain reaction, Research, Combination, protocols, Chain Reaction, primer, positive control, Primers, experimental protocols, independent, polymerase chain, 【제목키워드】 Laboratory, SARS-CoV-2 RNA, experiment, polymerase chain,