Abstract
We adopted the reverse-transcriptase-loop-mediated isothermal amplification (RT-LAMP) to detect severe acute respiratory syndrome coronavirus 2 (SARS-Cov-2) in patient samples. Two primer sets for genes N and Orf1ab were designed to detect SARS-CoV-2, and one primer set was designed to detect the human gene Actin. We collected prospective 138 nasopharyngeal swabs, 70 oropharyngeal swabs, 69 salivae, and 68 mouth saline wash samples from patients suspected to have severe acute respiratory syndrome (SARS) caused by SARS-CoV-2 to test the RT-LAMP in comparison with the gold standard technique reverse-transcription quantitative polymerase chain reaction (RT-qPCR). The accuracy of diagnosis using both primers, N5 and Orf9, was evaluated. Sensitivity and specificity for diagnosis were 96% (95% confidence interval [CI]: 87-99) and 85% (95% CI: 76-91) in 138 samples, respectively. Accurate diagnosis results were obtained only in nasopharyngeal swabs processed via extraction kit. Accurate and rapid diagnosis could aid coronavirus disease 2019 (COVID-19) pandemic management by identifying, isolating, and treating patients rapidly.
Keywords: RT-LAMP; coronavirus; molecular diagnosis.
【저자키워드】 coronavirus, RT-LAMP, Molecular diagnosis., 【초록키워드】 COVID-19, coronavirus disease, SARS-CoV-2, Coronavirus disease 2019, pandemic, Diagnosis, severe acute respiratory syndrome Coronavirus, Severe acute respiratory syndrome, Nasopharyngeal swab, polymerase chain reaction, RT-LAMP, specificity, RT-qPCR, Molecular diagnosis, Accuracy, Loop-mediated isothermal amplification, nasopharyngeal swabs, LAMP, management, Patient, gold, isothermal amplification, Quantitative, ORF1ab, Chain Reaction, acute respiratory syndrome, 95% CI, acute respiratory syndrome coronavirus, acute respiratory syndrome coronavirus 2, 95% confidence interval, gold standard, oropharyngeal swabs, primer, ORF1, quantitative polymerase chain reaction, Primers, human gene, polymerase chain, detect, collected, caused, primer set, evaluated, adopted, processed, 【제목키워드】 Brazil, SARS-CoV-2, diagnostic, detection, isothermal amplification, biofluid,