Background Countries across the globe have mobilized their armed forces in response to COVID-19, placing them at increased risk for viral exposure. Humoral responses to SARS-CoV-2 among military personnel serve as biomarkers of infection and provide a basis for disease surveillance and recognition of work-related risk factors. Methods Enzyme-linked immunosorbent assays (ELISA) were used to measure SARS-CoV-2 spike antigen-specific IgG in serum obtained from N = 988 US National Guard soldiers between April-June 2020. Occupational information, e.g. military operating specialty (MOS) codes, and demographic data were obtained via questionnaire. Plaque assays with live SARS-CoV-2 were used to assess serum neutralizing capacity for limited subjects (N = 12). Results The SARS-CoV-2 IgG seropositivity rate among the study population was 10.3% and significantly associated with occupation and demographics. Odds ratios were highest for those working in MOS 2T-Transportation (3.6; 95% CI 0.7–18) and 92F-Fuel specialist/ground and aircraft (6.8; 95% CI 1.5–30), as well as black race (2.2; 95% CI 1.2–4.1), household size ≥6 (2.5; 95% CI 1.3–4.6) and known COVID-19 exposure (2.0; 95% CI 1.2–3.3). Seropositivity tracked along major interstate highways and clustered near the international airport and the New York City border. SARS-CoV-2 spike IgG + serum exhibited low to moderate SARS-CoV-2 neutralizing capacity with IC 50s ranging from 1:15 to 1:280. In limited follow-up testing SARS-CoV-2 serum IgG levels remained elevated up to 7 months. Conclusions The data highlight increased SARS-CoV-2 seroprevalence among National Guard vs. the local civilian population in association with transportation-related occupations and specific demographics.
【초록키워드】 COVID-19, SARS-CoV-2, IgG, Biomarker, Biomarkers, Risk factors, Infection, Local, New York City, ELISA, serum, enzyme-linked immunosorbent assay, SARS-CoV-2 seroprevalence, Humoral response, enzyme-linked immunosorbent assays, International, Seropositivity, Disease surveillance, neutralizing capacity, Follow-up, humoral responses, information, moderate, questionnaire, SARS-CoV-2 IgG, spike IgG, SARS-CoV-2 spike, association, occupational, aircraft, International airport, demographics, Recognition, Armed Forces, 95% CI, increased risk, study population, subject, SARS-CoV-2 spike antigen, demographic data, Odds ratios, Black race, live SARS-CoV-2, serum IgG, household size, plaque assays, plaque, serum neutralizing capacity, highlight, Result, highest, globe, significantly, remained, elevated, exhibited, were used, MOS, 【제목키워드】 SARS-CoV-2, serum IgG,