Our understanding of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is still developing. We perform an observational study to investigate seroprevalence and immune responses in subjects professionally exposed to SARS-CoV-2 and their family members (155 individuals; ages 5–79 years). Seropositivity for SARS-CoV-2 Spike glycoprotein aligns with PCR results that confirm the previous infection. Anti-Spike IgG/IgM titers remain high 60 days post-infection and do not strongly associate with symptoms, except for fever. We analyze PBMCs from a subset of seropositive and seronegative adults. TLR7 agonist-activation reveals an increased population of IL-6 + TNF – IL-1β + monocytes, while SARS-CoV-2 peptide stimulation elicits IL-33, IL-6, IFNa2, and IL-23 expression in seropositive individuals. IL-33 correlates with CD4 + T cell activation in PBMCs from convalescent subjects and is likely due to T cell-mediated effects on IL-33-producing cells. IL-33 is associated with pulmonary infection and chronic diseases like asthma and COPD, but its role in COVID-19 is unknown. Analysis of published scRNAseq data of bronchoalveolar lavage fluid (BALF) from patients with mild to severe COVID-19 reveals a population of IL-33-producing cells that increases with the disease. Together these findings show that IL-33 production is linked to SARS-CoV-2 infection and warrant further investigation of IL-33 in COVID-19 pathogenesis and immunity. Our understanding of the immune response to SARS-CoV-2 infection is still incomplete. Here, the authors find that IL-33, produced during immune recall potentially by CD14 + monocytes, correlates with CD4 + T cell activation, anti-SARS-CoV-2 antibody titer, and disease severity in a cohort of convalescent individuals professionally exposed to the virus.
【저자키워드】 SARS-CoV-2, Interleukins, Epidemiology, 【초록키워드】 COVID-19, Monocytes, Asthma, coronavirus, immune response, Pathogenesis, severe COVID-19, Immunity, spike, IL-6, SARS-COV-2 infection, disease severity, Seroprevalence, spike glycoprotein, Infection, TLR7, peptide, severe acute respiratory syndrome Coronavirus, virus, CD4, Symptoms, observational study, immune, chronic disease, COPD, Adults, Cohort, T cell, PCR, COVID-19 pathogenesis, Bronchoalveolar lavage fluid, anti-SARS-CoV-2 antibody, anti-Spike IgG, cells, SARS-CoV-2 spike glycoprotein, immune responses, Fever, Patient, Antibody titer, IL-33, Seropositivity, Mild, PBMC, age, glycoprotein, recall, convalescent, expression, BALF, seropositive, seronegative, T cell activation, spike IgG, Pulmonary infection, TNF, IL-1β, PBMCs, Chronic diseases, acute respiratory syndrome, acute respiratory syndrome coronavirus, acute respiratory syndrome coronavirus 2, subject, CD14, convalescent individuals, seropositive individuals, IFNA2, IL-23, their family members, scRNAseq, Effect, Cell, convalescent individual, produced, the disease, increase, elicit, reveal, subset, days post-infection, Seropositivity for SARS-CoV-2, their family member, 【제목키워드】 COVID-19, expression, convalescent individual,