One of the most severe effects of coronavirus disease 2019 (COVID-19) is lung disorders such as acute respiratory distress syndrome. In the absence of effective treatments, it is necessary to search for new therapies and therapeutic targets. Platelets play a fundamental role in respiratory disorders resulting from viral infections, being the first line of defense against viruses and essential in maintaining lung function. The direct application of platelet lysate (PL) obtained from the platelet-rich plasma of healthy donors could help in the improvement of the patient due its anti-inflammatory, immunomodulatory, antifibrotic, and repairing effects. This work evaluates PL nebulization by analyzing its levels of growth factors and its biological activity on lung fibroblast cell cultures, besides describing a scientific basis for its use in this kind of pathology. The data of the work suggest that the molecular levels and biological activity of the PL are maintained after nebulization. Airway administration would allow acting directly on the lung tissue modulating inflammation and stimulating reparative processes on key structures such as the alveolocapillary barrier, improving the disease and sequels. The protocol developed in this work is a first step for the study of nebulized PL both in animal experimentation and in clinical trials.
【저자키워드】 COVID-19, Growth factors, Platelet, platelet-rich plasma, platelet lysate, nebulization, 【초록키워드】 coronavirus disease, viruses, Structure, Inflammation, pathology, Coronavirus disease 2019, therapy, protocol, acute respiratory distress syndrome, Anti-inflammatory, lung, clinical trials, Platelets, virus, viral infections, Growth factors, Viral, immunomodulatory, Lung function, Platelet, molecular, respiratory, therapeutic targets, acute respiratory distress, administration, platelet-rich plasma, nebulization, respiratory distress, healthy donors, syndrome, help, lung tissue, nebulized, lung disorders, growth factor, respiratory disorders, cell cultures, Defense, biological activity, antifibrotic, lung disorder, fibroblast, Effect, Effects, respiratory disorder, effective, healthy donor, resulting, evaluate, the patient, the disease, absence, acting, modulating, 【제목키워드】 rationale, proof, lysate, concept,