Graphical abstract Agrimonia pilosa (AP), Galla rhois (RG), and their mixture (APRG64) strongly inhibited SARS-CoV-2 by interfering with multiple steps of the viral life cycle including viral entry and replication. Furthermore, among 12 components identified in APRG64, three displayed strong antiviral activity, ursolic acid ( 1 ), quercetin ( 7 ), and 1,2,3,4,6-penta- O -galloyl- β – d -glucose ( 12 ). Molecular docking analysis showed these components to bind potently to the spike receptor-binding-domain (RBD) of the SARS-CoV-2 and its variant B.1.1.7. Taken together, these findings indicate APRG64 as a potent drug candidate to treat SARS-CoV-2 and its variants.
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【저자키워드】 COVID-19, SARS-CoV-2, COVID-19, Coronavirus disease 2019, SARS-CoV-2, Severe acute respiratory syndrome coronavirus 2, Antiviral agents, BSA, bovine serum albumin, ELISA, enzyme-linked immunosorbent assay, FBS, fetal bovine serum, IAV, influenza A virus, DMEM, Dulbecco’s modified Eagle’s medium, PFU, plaque-forming unit, hpi, hours post-infection, SEM, standard error of the mean, HCV, hepatitis C virus, HSV-1, Herpes simplex virus type 1,Agrimonia pilosa , APRG64, Galla rhois , AP, Agrimonia pilosa , APRG64, 50% EtOH aqueous extract mixture of AP and RG which ratio is 6 and 4, c.c., column chromatography, DENV-2, dengue virus 2, LC/MS, liquid chromatography/mass spectrometry, MEM, Eagle’s Minimum Essential Medium, ODS, octadecyl SiO2, RG, Galla rhois , SiO2, silica gel,
【저자키워드】 COVID-19, SARS-CoV-2, COVID-19, Coronavirus disease 2019, SARS-CoV-2, Severe acute respiratory syndrome coronavirus 2, Antiviral agents, BSA, bovine serum albumin, ELISA, enzyme-linked immunosorbent assay, FBS, fetal bovine serum, IAV, influenza A virus, DMEM, Dulbecco’s modified Eagle’s medium, PFU, plaque-forming unit, hpi, hours post-infection, SEM, standard error of the mean, HCV, hepatitis C virus, HSV-1, Herpes simplex virus type 1,