Alcohol-based disinfectant shortage is a serious concern in the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic. Acidic electrolyzed water (EW) with a high concentration of free available chlorine (FAC) shows strong antimicrobial activity against bacteria, fungi, and viruses. Here, we assessed the SARS-CoV-2-inactivating efficacy of acidic EW for use as an alternative disinfectant. The quick virucidal effect of acidic EW depended on the concentrations of contained-FAC. The effect completely disappeared in acidic EW in which FAC was lost owing to long-time storage after generation. In addition, the virucidal activity increased proportionately with the volume of acidic EW mixed with the virus solution when the FAC concentration in EW was same. These findings suggest that the virucidal activity of acidic EW against SARS-CoV-2 depends on the amount of FAC contacting the virus. Graphical abstract Image 1 Highlights • Acidic electrolyzed water (EW) shows virucidal activity against SARS-CoV-2. • Virucidal activity of acidic EW depends on free available chlorine (FAC). • Acidic solution without FAC does not inactivate SARS-CoV-2 in a 1-min reaction. • Large amounts of FAC are required to inactivate virus containing many proteins.
【저자키워드】 SARS-CoV-2, SARS-CoV-2, Severe acute respiratory syndrome coronavirus 2, FBS, fetal bovine serum, WHO, World Health Organization, disinfectant, acidic electrolyzed water, TCID50, 50% tissue culture infective dose, Free available chlorine, DDW, double-distilled water, EW, electrolyzed water, FAC, free available chlorine,