ABSTRACT The current study aims to develop a safe and highly immunogenic COVID-19 vaccine. The novel combination of a DNA vaccine encoding the full-length Spike (S) protein of SARS-CoV-2 and a recombinant S1 protein vaccine induced high level neutralizing antibody and T cell immune responses in both small and large animal models. More significantly, the co-delivery of DNA and protein components at the same time elicited full protection against intratracheal challenge of SARS-CoV-2 viruses in immunized rhesus macaques. As both DNA and protein vaccines have been proven safe in previous human studies, and DNA vaccines are capable of eliciting germinal center B cell development, which is critical for high-affinity memory B cell responses, the DNA and protein co-delivery vaccine approach has great potential to serve as a safe and effective approach to develop COVID-19 vaccines that provide long-term protection.
【저자키워드】 SARS-CoV-2, spike glycoprotein, protein vaccine, non-human primate, DNA vaccine, 【초록키워드】 neutralizing antibody, Vaccine, COVID-19 vaccine, immune response, spike, SARS-CoV-2 virus, animal models, B cell, Protein, DNA, T cell, Critical, rhesus macaques, Combination, Safe, Recombinant S1 protein, immunogenic, component, full-length, responses, approach, effective, Cell, immunized, memory B, develop, significantly, elicited, 【제목키워드】 COVID-19 vaccine, Protein, DNA, rhesus macaque, Combination, provide,