Abstract Background There is limited understanding of SARS-CoV-2 pathogenesis in African populations with a high burden of infectious disease comorbidities such as HIV. The kinetics, magnitude and duration of virus-specific antibodies and the underlying B cell responses in people living with HIV (PLWH) in sub-Saharan Africa have not been fully characterized. Methods We longitudinally followed SARS-CoV-2 infected individuals in Durban, KwaZulu-Natal, South Africa and characterized SARS-CoV-2 receptor binding domain-specific IgM, IgG and IgA antibodies weekly for a month, and then at 3 months post diagnosis. 7/30 (41.7%) were PLWH, 83% (25/30) of which were on ART and with full HIV suppression. Potency of convalescent plasma neutralization was determined using a live virus neutralization assay and antibody secreting cell population frequencies were determined by flow cytometry. Results Similar seroconversion rates, time to peak antibody titer, peak magnitude and durability of anti-SARS-CoV-2 IgM, IgG, IgA, were observed in HIV uninfected and PLWH with complete HIV suppression on ART. In addition, similar neutralization potency against an isolate of SARS-CoV-2, circulating at the time of sampling in the first wave of SARS-CoV-2 infections in South Africa was observed in both groups. Loss of IgA was significantly associated with age (p=0.023) and a previous diagnosis of TB (p=0.018). Conclusions Similar antibody response kinetics and neutralization potency in HIV negative and PLWH on stable ART in an African setting suggests that COVID-19 natural infections may confer comparable antibody immunity in these groups. This provides hope that COVID-19 vaccines will be effective in PLWH on stable ART.
【저자키워드】 antibodies, SARS-CoV-2, neutralization, South Africa, 【초록키워드】 COVID-19, convalescent plasma, IgG, IgM, HIV, COVID-19 vaccine, antibody, SARS-COV-2 infection, Antibody Response, Diagnosis, Comorbidity, Infectious disease, flow cytometry, SARS-CoV-2 receptor, Population, Neutralization assay, IgA, sub-Saharan Africa, African, ART, Antibody titer, age, natural infection, First wave, SARS-CoV-2 pathogenesis, PLWH, Live virus, anti-SARS-CoV-2 IgM, binding, Frequency, B cell response, neutralization potency, potency, seroconversion rates, circulating, antibody immunity, uninfected, Complete, Loss, effective, secreting cell, Result, significantly, addition, characterized, provide, was determined, magnitude, comparable, groups, IgA antibody, with HIV, in both groups, SARS-CoV-2 infected individual, 【제목키워드】 HIV, Antibody Response, Infection, African, Antiretroviral therapy, infected individual, South, uninfected,