Abstract
Thousands of breakthrough infections are confirmed after intramuscular (i.m.) injection of the approved vaccines against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Two major factors might contribute to breakthrough infections. One is the emergence of mutant variants of SARS-CoV-2, and the other is that i.m. injection has an inefficient ability to activate mucosal immunity in the upper respiratory tract. Here, we devised a dual-chambered nanocarrier that can codeliver the adjuvant CBLB502 with prefusion-spike (pre-S) onto a ferritin nanoparticle. This vaccine enabled enhanced systemic and local mucosal immunity in the upper and lower respiratory tract. Further, codelivery of CBLB502 with pre-S induced a Th1/Th2-balanced immunoglobulin G response. Moreover, the codelivery nanoparticle showed a Th1-biased cellular immune response as the release of splenic INF-γ was significantly heightened while the level of IL-4 was elevated to a moderate extent. In general, the developed dual-chambered nanoparticle can trigger multifaceted immune responses and shows great potential for mucosal vaccine development.
Keywords: CBLB502; codelivery; dual-chambered; ferritin nanocarrier; prefusion-spike; systemic and mucosal immunity.
【저자키워드】 CBLB502, codelivery, dual-chambered, ferritin nanocarrier, prefusion-spike, systemic and mucosal immunity., 【초록키워드】 SARS-CoV-2, Vaccine, Vaccine development, coronavirus, immune response, Immunity, Cellular immune response, ferritin, severe acute respiratory syndrome Coronavirus, Immunoglobulin G, Immunoglobulin, immune responses, breakthrough infections, Breakthrough infection, mutant, respiratory, upper respiratory tract, moderate, intramuscular, IL-4, mucosal, Lower respiratory tract, Trigger, INF-γ, acute respiratory syndrome, Factor, immunoglobulin G response, acute respiratory syndrome coronavirus, acute respiratory syndrome coronavirus 2, injection, upper and lower respiratory tract, prefusion, variants of SARS-CoV-2, significantly, approved, elevated, contribute, activate, inefficient, systemic and local, 【제목키워드】 SARS-CoV-2, systemic,