After the pandemic of COVID-19, neutralizing antibodies (NAbs) against SARS-CoV-2 have been developed for the prophylactic and therapeutic purposes. However, few methodologies are described in detail on how to rapidly and efficiently generate effective NAbs to SARS-CoV-2. Here, we integrated and optimized a strategically screening method for NAbs, which has enabled us to obtain SARS-CoV-2 receptor-binding domain (RBD) specific NAbs within 6 days, followed by additional 9 days for antibody production and function analysis. Using this method, we obtained 198 specific Abs against SARS-CoV-2 RBD from the blood samples of COVID-19 convalescent patients, and 96 of them showed neutralizing activity. At least 20% of these NAbs exhibited advanced neutralizing potency and high affinity, with the top two NAbs showing half-maximal inhibitory concentration (IC 50 ) to block authentic SARS-CoV-2 at 9.88 and 11.13 ng/ml, respectively. Altogether, our study provides an effective methodology with high applicable value for discovering potential preventative and therapeutic NAbs for the emerging infectious diseases.
【저자키워드】 SARS-CoV-2, Neutralizing antibodies, Spike protein, Receptor-binding domain (RBD), methodology, 【초록키워드】 neutralizing antibody, Diseases, Prophylactic, Neutralizing activity, RBD, therapeutic, Neutralizing, NAb, Analysis, antibody production, SARS-CoV-2 RBD, half-maximal inhibitory concentration, therapeutic purposes, NAbs, domain, blood sample, high affinity, pandemic of COVID-19, screening method, effective, COVID-19 convalescent patients, described, generate, exhibited, provide, 【제목키워드】 Screening, Rapid, application, efficient, System,