Background Striking similarities have been found between coronavirus disease 2019 (COVID-19) and anti-melanoma differentiation-associated gene 5 (MDA5) antibody (Ab)-related dermatomyositis, implying a shared autoinflammatory aberrance. Herein, we aim to investigate whether the anti-MDA5 Ab is present in COVID-19 and correlates with the severity and adverse outcome of COVID-19 patients. Methods and Findings We retrospectively recruited 274 adult inpatients with COVID-19 in this study, including 48, 164, and 62 cases of deaths, severe, and non-severe patients respectively. The anti-MDA5 Ab was determined by ELISA and verified by Western Blotting, which indicated that the positive rate of anti-MDA5 Ab in COVID-19 patients was 48.2% (132/274). The clinical and laboratory features, as well as outcomes between patients with positive and negative anti-MDA5 Ab were compared and we found that the anti-MDA5 Ab positive patients tended to represent severe disease (88.6% vs 66.9%, P <0.0001). We also demonstrated that the titer of anti-MDA5 Ab was significantly elevated in the non-survivals (5.95 ± 5.16 vs 8.22 ± 6.64, P =0.030) and the positive rate was also higher than that in the survivals (23.5% vs 12.0%, P =0.012). Regarding severe COVID-19 patients, we found that high titer of anti-MDA5 Ab (≥10.0 U/mL) was more prevalent in the non-survivals (31.2% vs 14.0%, P =0.006). Moreover, a dynamic analysis of anti-MDA5 Ab was conducted at different time-points of COVID-19, which revealed that early profiling of anti-MDA5 Ab could distinguish severe patients from those with non-severe ones. Conclusions Anti-MDA5 Ab was prevalent in the COVID-19 patients and high titer of this antibody is correlated with severe disease and unfavorable outcomes.
【저자키워드】 COVID-19, Innate immunity, acute respiratory distress syndrome (ARDS), Autoimmune, anti-melanoma differentiation-associated gene 5 (MDA5) antibody, dermatomyositis, 【초록키워드】 coronavirus disease, antibody, severity, outcome, ELISA, adverse outcome, outcomes, survival, MDA5, Patient, Severe patient, positive rate, COVID-19 patients, Analysis, severe disease, deaths, similarity, COVID-19 patient, Laboratory features, Inpatient, severe COVID-19 patients, finding, positive, prevalent, significantly, recruited, indicated, elevated, conducted, was determined, demonstrated, correlated, positive patient, Blotting, non-severe patient, with COVID-19, 【제목키워드】 clinical, assessment, value, Presence,