Rationale Systemic activation of procoagulant and inflammatory mechanisms has been implicated in the pathogenesis of COVID-19. Knowledge of activation of these host response pathways in the lung compartment of COVID-19 patients is limited. Objectives To evaluate local and systemic activation of coagulation and interconnected inflammatory responses in critically ill COVID-19 patients with persistent acute respiratory distress syndrome. Methods Paired bronchoalveolar lavage fluid and plasma samples were obtained from 17 patients with COVID-19 related persistent acute respiratory distress syndrome (mechanical ventilation > 7 days) 1 and 2 weeks after start mechanical ventilation and compared with 8 healthy controls. Thirty-four host response biomarkers stratified into five functional domains (coagulation, complement system, cytokines, chemokines and growth factors) were measured. Measurements and Main Results In all patients, all functional domains were activated, especially in the bronchoalveolar compartment, with significantly increased levels of D-dimers, thrombin-antithrombin complexes, soluble tissue factor, C1-inhibitor antigen and activity levels, tissue type plasminogen activator, plasminogen activator inhibitor type I, soluble CD40 ligand and soluble P-selectin (coagulation), next to activation of C3bc and C4bc (complement) and multiple interrelated cytokines, chemokines and growth factors. In 10 patients in whom follow-up samples were obtained between 3 and 4 weeks after start mechanical ventilation many bronchoalveolar and plasma host response biomarkers had declined. Conclusions Critically ill, ventilated patients with COVID-19 show strong responses relating to coagulation, the complement system, cytokines, chemokines and growth factors in the bronchoalveolar compartment. These results suggest a local pulmonary rather than a systemic procoagulant and inflammatory “storm” in severe COVID-19.
【저자키워드】 COVID-19, innate immune response, Coagulation, persistent ARDS, bronchoalveolar space, 【초록키워드】 Biomarker, Cytokines, severe COVID-19, mechanical ventilation, lung, Local, complement, host response, chemokine, P-selectin, Antigen, Growth factors, Critically ill, Bronchoalveolar lavage fluid, response, Patient, pathway, plasma, Follow-up, inhibitor, patients, mechanism, acute respiratory distress, Inflammatory response, Inflammatory, COVID-19 patient, systemic, Activation, tissue, type I, domain, rationale, syndrome, pathogenesis of COVID-19, healthy controls, growth factor, CD40 ligand, C1-Inhibitor, measurement, objective, FIVE, procoagulant, bronchoalveolar, plasma sample, significantly increased, Result, evaluate, functional, activated, complexes, implicated, were measured, stratified, declined, patients with COVID-19, ventilated patient, with COVID-19, 【제목키워드】 pulmonary, response, Innate, Ill,