Abstract
The patterns of humoral and cellular responses to SARS-CoV-2 were studied in Swedish primary health care workers (n = 156) for 6 months during the Covid-19 pandemic. Serum IgA and IgG to SARS-CoV-2, T-cell proliferation and cytokine secretion, demographic and clinical data, PCR-verified infection, and self-reported symptoms were monitored. The multivariate method OPLS-DA was used to identify immune response patterns coupled to protection from Covid-19. Contracting Covid-19 was associated with SARS-CoV-2-specific neutralizing serum IgG, T cell, IFN-γ, and granzyme B responses to SARS-CoV-2, self-reported typical Covid-19 symptoms, male sex, higher BMI, and hypertension. Not contracting Covid-19 was associated with female sex, IgA-dominated, or no antibody responses to SARS-CoV-2, airborne allergy, and smoking. The IgG-responders had SARS-CoV-2-specific T-cell responses including a cytotoxic CD4+ T-cell population expressing CD25, CD38, CD69, CD194, CD279, CTLA-4, and granzyme B. IgA-responders with no IgG response to SARS-CoV-2 constituted 10% of the study population. The IgA responses were partially neutralizing and only seen in individuals who did not succumb to Covid-19. To conclude, serum IgG-dominated responses correlated with T-cell responses to SARS-CoV-2 and PCR-confirmed Covid-19, whereas IgA-dominated responses correlated with not contracting the infection.
Keywords: Cytotoxic T cell; IgA; Neutralizing antibodies; Primary health care; SARS-CoV-2.
【저자키워드】 Neutralizing antibodies, SARS-CoV-2., Primary Health Care, IgA, Cytotoxic T cell, 【초록키워드】 SARS-CoV-2, IgG, immune response, pandemic, Antibody Response, T-cell Response, Infection, Sex, smoking, hypertension, Symptoms, CTLA-4, serum, T cell, Health, response, female, Health care worker, allergy, Neutralizing, T-cell, BMI, CD25, CD38, IFN-γ, cellular response, granzyme B, Male sex, CD69, humoral, proliferation, IgG response, Contracting, PCR-confirmed, study population, Clinical data, individual, serum IgG, cytotoxic, IgA response, CD4+ T-cell, cytokine secretion, self-reported symptom, identify, was used, correlated, Swedish, expressing, CD279, SARS-CoV-2-specific T-cell response, 【제목키워드】 COVID-19, anti-SARS-CoV-2, serum, Primary Health Care, Health care worker, PROTECT, appear,