Abstract
COVID-19 pandemic has resulted in an unprecedented global public health crisis. It is obvious that SARS-CoV-2 vaccine is needed to control the global COVID-19 public health crisis. Since obvious advantages including fast manufacturing speed, potent immunogenicity and good safety profile, six mRNA vaccines have been used to prevent SARS-CoV-2 infections in clinic with lipid nanoparticles (LNP) formulation via intramuscular injection. In this work, we first constructed RBD-encoding mRNA (RBD-mRNA) formulated in liposomes (LPX/RBD-mRNA) and investigated the influence of administration routes on the immunogenicity. LPX/RBD-mRNA can express RBD in vivo and successfully induced SARS-CoV-2 RBD specific antibodies in the vaccinated mice, which efficiently neutralized SARS-CoV-2 pseudotyped virus. Moreover, the administration routes were found to affect the virus neutralizing capacity of sera derived from the immunized mice and the types (Th1-type and Th2-type) of cellular immune responses. This study indicated that liposome-based RBD-mRNA vaccine with optimal administration route might be a potential candidate against SARS-CoV-2 infection with good efficacy and safety.
Keywords: Administration route; Liposomes; Receptor-binding domain; SARS-CoV-2; mRNA vaccines.
【저자키워드】 SARS-CoV-2, liposomes, mRNA vaccines, Receptor-binding domain, Administration route, 【초록키워드】 COVID-19, Vaccine, pandemic, mRNA vaccine, SARS-COV-2 infection, public health crisis, virus, SARS-CoV-2 vaccine, LNP, mRNA vaccines, mice, RBD, mRNA, sera, Efficacy and safety, neutralizing capacity, in vivo, safety profile, Lipid nanoparticle, administration, Liposome, Intramuscular injection, SARS-CoV-2 RBD, global public health, cellular immune responses, Express, SARS-CoV-2 pseudotyped virus, immunized mice, Affect, Prevent, neutralized, indicated, investigated, specific antibody, Th2-type, 【제목키워드】 mRNA vaccine, Liposome,