Abstract
Here, we describe the use of the artificial antigen-presenting cell (aAPC) system for the verification of T-cell epitopes. We purify and activate CD8 + T cells from blood samples from HLA-A2 that are negative for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). CD8 + T cells are combined with peptide-loaded T2-A2 cells, which are then stained with a SARS-CoV-2-specific MHC-1 tetramer to identify specific HLA-A2-restricted T-cell epitopes. The use of aAPC and healthy donors means that only BSL2 lab conditions are needed. For details of the use and implementation of this protocol, please refer to Deng et al. (2021).
Keywords: Cell Biology; Cell isolation; Immunology; Microbiology.
【저자키워드】 immunology, Cell Biology, Cell isolation, Microbiology., 【초록키워드】 immunology, SARS-CoV-2, coronavirus, Microbiology, protocol, T cells, peptide, severe acute respiratory syndrome Coronavirus, CD8, Cell Biology, Epitopes, T cell, cells, implementation, respiratory, T-cell epitopes, antigen-presenting cell, acute respiratory syndrome, acute respiratory syndrome coronavirus, acute respiratory syndrome coronavirus 2, Blood samples, blood sample, verification, aAPC, BSL2, lab, healthy donor, identify, condition, activate, 【제목키워드】 COVID-19, Infectious disease, CD8, optimization, T cell activation, condition,