Abstract
Immunosuppressed patients with inflammatory bowel disease (IBD) generate lower amounts of SARS-CoV-2 spike antibodies after mRNA vaccination than healthy controls. We assessed SARS-CoV-2 spike S1 receptor binding domain-specific (S1-RBD-specific) B lymphocytes to identify the underlying cellular defects. Patients with IBD produced fewer anti-S1-RBD antibody-secreting B cells than controls after the first mRNA vaccination and lower amounts of total and neutralizing antibodies after the second. S1-RBD-specific memory B cells were generated to the same degree in IBD and control groups and were numerically stable for 5 months. However, the memory B cells in patients with IBD had a lower S1-RBD-binding capacity than those in controls, which is indicative of a defect in antibody affinity maturation. Administration of a third shot to patients with IBD elevated serum antibodies and generated memory B cells with a normal antigen-binding capacity. These results show that patients with IBD have defects in the formation of antibody-secreting B cells and affinity-matured memory B cells that are corrected by a third vaccination.
Keywords: Adaptive immunity; COVID-19; Immunology; Inflammatory bowel disease; Memory.
【저자키워드】 COVID-19, immunology, Adaptive immunity, memory, Inflammatory bowel disease, 【초록키워드】 neutralizing antibody, mRNA vaccination, vaccination, antibody, B cell, Patient, Control, serum antibody, B lymphocyte, memory B cell, IBD, SARS-CoV-2 spike, cellular, Receptor binding, antibody affinity, control group, maturation, healthy controls, bowel, controls, produced, identify, generate, elevated, 【제목키워드】 SARS-CoV-2, Patient, Cell, memory B,