Abstract
Immunogenicity following inactivated SARS-CoV-2 vaccination among solid organ transplant recipients has not been assessed. Seventy-five patients (37 kidney transplant [KT] recipients and 38 healthy controls) received two doses, at 4-week intervals, of an inactivated whole-virus SARS-CoV-2 vaccine. SARS-CoV-2-specific humoral (HMI) and cell-mediated immunity (CMI) were measured before, 4 weeks post-first dose, and 2 weeks post-second dose. The median (IQR) age of KT recipients was 50 (42-54) years and 89% were receiving calcineurin inhibitors/mycophenolate/corticosteroid regimens. The median (IQR) time since transplant was 4.5 (2-9.5) years. Among 35 KT patients, the median (IQR) of anti-RBD IgG level measured by CLIA after vaccination was not different from baseline, but was significantly lower than in controls (2.4 [1.1-3.7] vs. 1742.0 [747.7-3783.0] AU/ml, p < .01) as well as percentages of neutralizing antibody inhibition measured by surrogate viral neutralization test (0 [0-0] vs. 71.2 [56.8-92.2]%, p < .01). However, the median (IQR) of SARS-CoV-2 mixed peptides-specific T cell responses measured by ELISpot was significantly increased compared with baseline (30 [4-120] vs. 12 [0-56] T cells/10 6 PBMCs, p = .02) and not different from the controls. Our findings revealed weak HMI but comparable CMI responses in fully vaccinated KT recipients receiving inactivated SARS-CoV-2 vaccination compared to immunocompetent individuals (Thai Clinical Trials Registry, TCTR20210226002).
Keywords: coronavirus; immunocompromised; neutralizing antibody; receptor binding domain; renal transplant; spike protein; vaccine.
【저자키워드】 neutralizing antibody, Vaccine, coronavirus, Spike protein, Receptor binding domain, Immunocompromised, Renal transplant, 【초록키워드】 CLIA, SARS-CoV-2, Vaccine, vaccination, immunogenicity, SARS-CoV-2 vaccine, clinical, response, Patient, Control, age, Neutralizing, ELISPOT, viral neutralization, registry, patients, T cell response, kidney transplant, SARS-CoV-2 vaccination, Cell-mediated immunity, inactivated, solid organ transplant, dose, PBMCs, Receptor binding, humoral, Anti-RBD IgG, individual, regimens, healthy controls, doses, renal, recipient, significantly lower, intervals, CMI, controls, significantly increased, receiving, median, comparable, not different, were measured, baseline, IQR, percentage, the median, 【제목키워드】 COVID-19 vaccine, immunization, inactivated, kidney transplant recipient, humoral, cell-mediated immune response,