Abstract
We evaluated the effect of vaccination with anti-COVID-19 vaccine EpiVacCorona on serum antimicrobial activity, formation of specific IgG antibodies, and expression of some antimicrobial peptides. Antimicrobial activity of the serum from 55 volunteers towards S. aureus cells was measured spectrophotometrically; IgG-antibodies against SARS-CoV-2 antigen were assayed by ELISA; expression of genes encoding antimicrobial peptides LL37, HBD1, and HBD2 was evaluated by PCR with reverse transcription. Total antimicrobial serum activity and activity of its low-molecular-weight fraction containing antimicrobial peptides demonstrated an inverse correlation. Both activities after vaccination increased in case of low initial values, but decreased in case of high initial values. The vector of change of specific IgG antibodies to coronavirus inversely correlated with the vector of change of activity of antimicrobial peptide fraction. The expression of genes of antimicrobial peptides LL37, HBD1, and HBD2 looked like normal distribution depending on activities of the antimicrobial peptides in the corresponding sera.
Keywords: EpiVacCorona; antimicrobial activity; antimicrobial peptides; serum; vaccination.
【저자키워드】 vaccination, serum, antimicrobial peptides, antimicrobial activity, EpiVacCorona, 【초록키워드】 Vaccine, coronavirus, vaccination, antimicrobial, activity, PCR, sera, peptides, reverse transcription, SARS-CoV-2 antigen, expression, specific IgG antibodies, fraction, inverse correlation, antimicrobial peptide, Normal distribution, Volunteer, Cell, initial, was measured, evaluated, demonstrated, correlated, Total, expression of gene, specific IgG antibody, 【제목키워드】 antimicrobial, activity,