Abstract
This protocol enables the testing of drugs against infection of epithelial cells with SARS-CoV-2 (severe acute respiratory syndrome coronavirus-2), using pseudo-typed replication deficient vesicular stomatitis virus particles (pp-VSV) presenting the SARS-CoV-2 spike protein. After treating human volunteers with amitriptyline, an approved antidepressant and inhibitor of the acid sphingomyelinase, freshly isolated nasal epithelial cells were infected ex vivo and infection levels were quantified. This protocol offers the possibility to rapidly test the efficacy of potential drugs in the fight against COVID-19. For complete details on the use and execution of this protocol, please refer to Carpinteiro et al. (2020).
Keywords: Cell isolation; Cell-based assays.
【저자키워드】 Cell isolation, Cell-based assays., 【초록키워드】 COVID-19, SARS-CoV-2, Efficacy, protocol, Infection, drug, severe acute respiratory syndrome Coronavirus, Spike protein, Particle, Replication, Severe acute respiratory syndrome, SARS-CoV-2 spike protein, epithelial cells, respiratory, inhibitor, vesicular stomatitis virus, Coronavirus-2, antidepressant, epithelial cell, vesicular stomatitis, Ex vivo, acute respiratory syndrome, amitriptyline, acute respiratory syndrome coronavirus, acute respiratory syndrome coronavirus-2, infection levels, stomatitis, offer, Volunteer, Complete, were infected, assays, approved, presenting, quantified, infection level, nasal epithelial cell, the SARS-CoV-2, 【제목키워드】 Efficacy, SARS-COV-2 infection, sphingolipid, evaluate, drugs targeting, nasal epithelial cell,