Abstract
The current coronavirus disease 2019 (COVID-19) pandemic is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), a novel coronavirus genetically close to SARS-CoV. To investigate the effects of previous SARS-CoV infection on the ability to recognize and neutralize SARS-CoV-2, we analyzed 20 convalescent serum samples collected from individuals infected with SARS-CoV during the 2003 SARS outbreak. All patient sera reacted strongly with the S1 subunit and receptor binding domain (RBD) of SARS-CoV; cross-reacted with the S ectodomain, S1, RBD, and S2 proteins of SARS-CoV-2; and neutralized both SARS-CoV and SARS-CoV-2 S protein-driven infections. Analysis of antisera from mice and rabbits immunized with a full-length S and RBD immunogens of SARS-CoV verified cross-reactive neutralization against SARS-CoV-2. A SARS-CoV-derived RBD from palm civets elicited more potent cross-neutralizing responses in immunized animals than the RBD from a human SARS-CoV strain, informing strategies for development of universal vaccines against emerging coronaviruses.
【초록키워드】 COVID-19, coronavirus disease, SARS-CoV-2, Coronaviruses, Vaccine, coronavirus, pandemic, SARS-CoV, neutralization, Novel coronavirus, Receptor binding domain, infections, outbreak, mice, RBD, immunogen, response, convalescent serum, Antisera, cross-reactive, acute respiratory syndrome, individual, SARS-CoV infection, patient sera, ectodomain, SARS-CoV-2 S, full-length S, Effect, palm civet, S2 protein, neutralize, immunized, neutralized, the S1 subunit, analyzed, collected, caused, 2003 SARS, recognize, the RBD, elicited, cross-neutralizing, 【제목키워드】 SARS-CoV-2, neutralization, serum antibody, immunized, SARS patient,