Abstract
This protocol is a comprehensive guide to phage display-based selection of virus neutralizing V H antibody domains. It details three optimized parts including (1) construction of a large-sized (theoretically > 10 11 ) naïve human antibody heavy chain domain library, (2) SARS-CoV-2 antigen expression and stable cell line construction, and (3) library panning for selection of SARS-CoV-2-specific antibody domains. Using this protocol, we identified a high-affinity neutralizing human V H antibody domain, V H ab8, which exhibits high prophylactic and therapeutic efficacy. For complete details on the use and execution of this protocol, please refer to Li et al. (2020).
Keywords: Antibody; Cell culture; High Throughput Screening; Immunology; Microbiology; Molecular Biology; Protein expression and purification.
【저자키워드】 immunology, Microbiology, Molecular biology, antibody, Cell culture, High Throughput Screening, Protein expression and purification, 【초록키워드】 protocol, Prophylactic, virus, Neutralizing, SARS-CoV-2 antigen, expression, domains, domain, therapeutic efficacy, naïve, throughput, cell line, purification, Complete, exhibit, 【제목키워드】 SARS-CoV-2 neutralizing antibody, domain,