Abstract
Convalescent coronavirus disease 2019 (COVID-19) subjects who receive BNT162b2 develop robust antibody responses against SARS-CoV-2. However, our understanding of the clonal B cell response pre- and post-vaccination in such individuals is limited. Here we characterized B cell phenotypes and the BCR repertoire after BNT162b2 immunization in two convalescent COVID-19 subjects. BNT162b2 stimulated many B cell clones that were under-represented during SARS-CoV-2 infection. In addition, the vaccine generated B cell clusters with >65% similarity in CDR3 V H and V L region consensus sequences both within and between subjects. This result suggests that the CDR3 region plays a dominant role adjacent to heavy and light chain V/J pairing in the recognition of the SARS-CoV-2 spike protein. Antigen-specific B cell populations with homology to published SARS-CoV-2 antibody sequences from the CoV-AbDab database were observed in both subjects. These results point towards the development of convergent antibody responses against the virus in different individuals.
Keywords: Antibodies; B cells; COVID19; SARS CoV2.
【저자키워드】 antibodies, COVID19, B cells, SARS CoV2, 【초록키워드】 COVID-19, coronavirus disease, SARS-CoV-2, SARS-COV-2 infection, Antibody Response, virus, database, immunization, Population, Spike protein, B cell, BNT162b2, SARS-CoV-2 antibody, Cluster, phenotype, convalescent, BCR, consensus sequence, similarity, B cell response, subject, individual, sequence, homology, clone, CDR3, dominant, stimulated, develop, addition, characterized, subjects, individuals, the vaccine, receive, CDR3 region, robust antibody response, the SARS-CoV-2, 【제목키워드】 COVID-19, BNT162b2 vaccine, convalescent, subject, homology, CDR3, spike-specific antibody response, receiving,