The COVID-19 pandemic, and future pandemics, require diagnostic tools to track disease spread and guide the isolation of (a)symptomatic individuals. Lateral-flow diagnostics (LFDs) are rapid and of lower cost than molecular (genetic) tests, with current LFDs using antibodies as their recognition units. Herein, we develop a prototype flow-through device (related, but distinct to LFDs), utilizing N- acetyl neuraminic acid-functionalized, polymer-coated, gold nanoparticles as the detection/capture unit for SARS-COV-2, by targeting the sialic acid-binding site of the spike protein. The prototype device can give rapid results, with higher viral loads being faster than lower viral loads. The prototype’s effectiveness is demonstrated using spike protein, lentiviral models, and a panel of heat-inactivated primary patient nasal swabs. The device was also shown to retain detection capability toward recombinant spike proteins from several variants (mutants) of concern. This study provides the proof of principle that glyco-lateral-flow devices could be developed to be used in the tracking monitoring of infectious agents, to complement, or as alternatives to antibody-based systems.
【저자키워드】 COVID-19, SARS-CoV-2, glycobiology, Nanoparticles, glycans, polymers, lateral flow, flow-through, rapid diagnostics, 【초록키워드】 antibody, COVID-19 pandemic, Genetic, diagnostic, complement, diagnostics, Spike protein, Viral, Viral load, Patient, Effectiveness, Isolation, Pandemics, gold, mutants, molecular, disease spread, Gold nanoparticle, Recombinant spike protein, Device, Spike proteins, infectious agents, sialic acid, LFD, principle, viral loads, symptomatic individuals, recombinant spike proteins, several variants, proof, alternatives, heat, nasal swabs, heat-inactivated, shown, develop, provide, faster, the spike protein, demonstrated, individuals, lentiviral, several variant, 【제목키워드】 detection,