Abstract
Reflecting on the past three years and the coronavirus disease 19 (COVID-19) pandemic, varying global tactics offer insights into the most effective public-health responses. In the US, specifically, rapid and widespread testing was quickly prioritized to lower restrictions sooner. Essentially, only two types of COVID-19 diagnostic tests were publicly employed during the peak pandemic: the rapid antigen test and reverse transcription polymerase chain reaction (RT-PCR). However, neither test ideally suited the situation, as rapid antigen tests are far too inaccurate, and RT-PCR tests require skilled personnel and sophisticated equipment, leading to long wait times. Loop-mediated isothermal amplification (LAMP) is another exceptionally accurate nucleic acid amplification test (NAAT) that offers far quicker time to results. However, RT-LAMP COVID-19 tests have not been embraced as extensively as rapid antigen tests or RT-PCR. This review will investigate the performance of current RT-LAMP-based COVID-19 tests and summarize the reasons behind the hesitancy to embrace RT-LAMP instead of RT-PCR. We will also look at other LAMP platforms to explore possible improvements in the accuracy and portability of LAMP, which could be applied to COVID-19 diagnostics and future public-health outbreaks.
Keywords: RT-LAMP; RT-PCR; SARS-CoV-2; loop-mediated isothermal amplification; nucleic acid amplification test (NAAT); rapid antigen test.
【저자키워드】 SARS-CoV-2, RT-PCR, RT-LAMP, rapid antigen test, Loop-mediated isothermal amplification, nucleic acid amplification test (NAAT), 【초록키워드】 COVID-19, coronavirus disease, pandemic, equipment, diagnostic test, diagnostics, Antigen, improvement, Outbreaks, rapid antigen test, Accuracy, NAAT, reverse transcription, nucleic acid amplification, platform, RT-PCR test, isothermal, COVID-19 test, offer, widespread, responses, effective, polymerase chain, Reflecting, 【제목키워드】 COVID-19, PCR, Result,